Figure 2

Mutp53 plays an important role in HCC arsenic trioxide resistance. (A) Knockdown of mutp53 in HCC resistant cells. (B) MTT assays were performed after cells were treated with arsenic trioxide for 48 h. ***: P < 0.001 versus negative siRNA-transfected cells. (C) Cells were treated with arsenic trioxide (2 μM) for 48 h before performing apoptosis assay. **, P < 0.01 versus negative siRNA-transfected cells. Histograms represent averages of three independent experiments. (D) Cells were treated with arsenic trioxide (2 μM) for 24 h after siRNA transfection (48 h). The target proteins were detected by Western blot analyses. (E) One representative Western blot from three independent experiments demonstrates the accumulation of the V5-tagged exogenous mutant p53 in each cell lines. (F) MTT assays were performed after cells were treated with arsenic trioxide for 48 h. ***: P < 0.001 versus vector control cell lines.