Figure 3

Mutp53 contributed to the increased metastatic potential of HCC resistant cells. (A) The migration and invasive abilities of HCC parental and arsenic trioxide resistant cells were determined by trans-well assays in chambers coated with matrigel (for invasion assays) or without matrigel (for migration assays). Left panels: representative images; right panels: quantifications of average number of cells/field. *P < 0.05, **P < 0.01, ***P < 0.001, two-way ANOVA with Bonferroni post-test. (B) Results of the migration and invasion assays for the HepG2/As and SMMC7721/As cells transfected with p53 siRNA or control siRNA. (**P < 0.01, ***P < 0.001, two-way ANOVA with Bonferroni post-test). (C) Single and merged images were taken to show immunofluorescence staining of N-cadherin (green) and vimentin (red) accompanied by the cell nucleus (blue) stained by DAPI.