Figure 4
From: RPN2-mediated glycosylation of tetraspanin CD63 regulates breast cancer cell malignancy
Effect of RPN2 or CD63 knockdown on MDR1 localization in breast cancer cells. A) MCF7-ADR cells were grown and transiently transfected with N.C. (upper panel), RPN2 (center panel) or CD63 siRNAs (right panel). After 2 days in culture, the immunofluorescence of MDR1 (Red) was detected. The blue color indicates nuclei. The size bar indicates 20 μm. B) MCF7-ADR cells were grown and transiently transfected with the Control (N.C., upper panels), RPN2 (meddle panels) or CD63 siRNAs (lower panels). After 2 days in culture, immunofluorescence of CD63 (Green) and MDR1 (Red) was performed. The blue color indicates nuclei. Co-localization of CD63 and MDR1-positive cells appears as yellow in the overlay image. The size bar indicates 50 μm. C) MCF7-ADR cells were grown and transiently transfected with N.C.. After 2 days in culture, the immunofluorescence of CD63 (Green) and MDR1 (Red) was detected. The blue color indicates nuclei. Co-localization of CD63 and MDR1-positive cells appears as yellow in the overlay image. The size bar indicates 20 μm.