Figure 2From: Estrogen promotes stemness and invasiveness of ER-positive breast cancer cells through Gli1 activationER expression in MCF-7, HCC1428, MDA-MB-231 and BT549 cells. (A) ER protein levels were analyzed using western blotting. β-Actin levels were measured as a loading control. (B) Histograms illustrate ER protein expression relative to that of β-actin. All data corresponded to the mean ± SD of three independent experiments. (C) Immunofluorescence staining of ER in MCF-7, HCC1428, MDA-MB-231 and BT549 cells. Green represents ER staining. Blue signals represent nuclear DNA staining with DAPI. Scale bars indicate 25 μm.Back to article page