Figure 5From: Estrogen promotes stemness and invasiveness of ER-positive breast cancer cells through Gli1 activationEstrogen increased the proliferation of ER-positive breast cancer cells. (A) Colony formation assays in MCF-7 and HCC1428 cells. MCF-7 and HCC1428 cells were transfected with vehicle control shRNA (shVEC), shGli1-1 or shGli1-2 in the absence or presence of E2. (B & C) The number of colonies was counted under a microscope. Error bars represent the SEM. **, ## indicate significant differences from the control (p < 0.001).Back to article page