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Figure 2 | Molecular Cancer

Figure 2

From: Alpha-mangostin inhibits intracellular fatty acid synthase and induces apoptosis in breast cancer cells

Figure 2

Apoptotic effect of α-mangostin in MCF-7 and MDA-MB-231 cells. A α-Mangostin induced apoptosis in MCF-7 and MDA-MB-231 cells as assessed by PARP cleavage (note intact PARP at 116 kDa and its cleavage product at 89 kDa) B MCF-7 and MDA-MB-231 cells were double-stained with annexin V and PI and analyzed by flow cytometry. The gate setting distinguished between living (bottom left), necrotic (top left), early apoptotic (bottom right), and late apoptotic (top right) cells. C The expression of Bax and Bcl-2 were determined by immunoblotting. MCF-7 and MDA-MB-231 cells were treated with α-mangostin at different concentrations for 24 h and equal amounts of lysates were immunoblotted with the corresponding antibody. Blots were reprobed for GAPDH as a loading control. In all cases, shown gels were representative of those obtained from at least two independent experiments.

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