Figure 5

Effects of α-mangostin on PI3K/AKT and MAPK/ERK1/2 signaling pathways in breast cancer cells. A α-Mangostin induced FAS blockade led to down-regulate p-AKT in both MCF-7 and MDA-MB-231 cells. B α-Mangostin induced ERK1/2 activation in both MCF-7 and MDA-MB-231 cells. Cells were treated with α-mangostin at different concentrations for 24 h and equal amounts of lysates were immunoblotted with the corresponding antibody. Blots were reprobed for GAPDH as a loading control. In all cases, shown gels were representative of those obtained from at least two independent experiments.