The expression of Notch1 in MG63 and Saos-2 cell lines and their sensitivity to cisplatin. Real-time PCR revealed that Saos-2 cells had approximately 7 fold higher Notch1 expression than MG63 cells (A). We further carried out Western Blot and immunocytochemistry to determine the protein expression in MG63 and Saos-2. The level of Notch1 in Saos-2 is significantly higher than MG63 (error bar means s.d.) (B and C). Both two cell lines showed dose and time dependent response to cisplatin (D). Saos-2 performed significantly higher sensitivity to cisplatin (E). (*P < 0.05, **P < 0.01).