Inhibition of Notch1 signaling pathway decreased the expression and/or activity of Caspase3, Caspase8 and Caspase9. In the two cell lines, realtime PCR indicated that the gene expression of Caspase3, Caspase8 and Caspase9 were reduced to some extent after a simple DAPT treatment for 24 hours. Further reduction was observed with cisplatin subsequent treatment for 12 hours (A, B, C and D). Simultaneously, western blot was carried out to detect the protein expression of Pro-Casp3, Cleaved-Casp3, Casp8, Pro-Casp9 and Cleaved-Casp9. The expression of these several proteins significantly decreased after DAPT treatment in MG63 and Saos-2 cells. Further, such difference between two groups could be obviously magnified after cisplatin treatment (E, F, G and H). (*P < 0.05; **P < 0.01).