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Figure 2 | Molecular Cancer

Figure 2

From: Colon cancer-derived oncogenic EGFR G724S mutant identified by whole genome sequence analysis is dependent on asymmetric dimerization and sensitive to cetuximab

Figure 2

Pharmacological effects of cetuximab against oncogenic G719S and G724S mutants in vitro and in vivo. (A) Cetuximab suppresses the growth of Ba/F3 cells dependent upon the G719S and G724S mutants, but not control cells. Ba/F3 cells transformed with the indicated EGFR mutants were treated with cetuximab at the concentrations indicated and assayed for viability after 72 hours of drug treatment. The results are indicated as mean +/- SD of sextuplicate wells and are representative of three independent experiments (B and C) Cetuximab is effective against SW48 (EGFR G719S mutant)-induced tumors but not HCT8 (KRAS G13D mutant) induced-tumors in xenografted mice. BALB/c-nu/nu mice (6–8 weeks of age) were injected subcutaneously to the flank with 0.5 ~ 1x107 SW48 or HCT8 cells in 150 ~ 200 μl of PBS. Tumor sizes were measured two times a week using a Vernier caliper and tumor volumes were calculated according to the formula of (short diameter)2 x (long diameter)/2. When tumor volume reached around 100 ~ 150 mm3, mice were randomized into each group. After confirming that mean tumor volumes were not statistically different between two groups, mice were administered either with PBS or cetuximab (1 mg/mouse) intra-peritoneally twice a week.

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Molecular Cancer

ISSN: 1476-4598