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Figure 2 | Molecular Cancer

Figure 2

From: mTOR inhibition and levels of the DNA repair protein MGMT in T98G glioblastoma cells

Figure 2

TMZ combined with KU0063794 decreases MGMT protein levels compared to treatments with KU0063794 alone, but an increase in overall MGMT protein levels is still observed. T98G cells were incubated in the absence (lane 1) or presence of TMZ and KU0063794 for 12 hours (lane 2), 24 hours (lanes 3), 48 hours (lane 4) or 72 hours (lane 5). A Proteins were visualised by Western blotting using the antiserum indicated. B. MGMT protein levels in (A) were quantified and expressed relative to the α tubulin loading control. Error bars are the SE (n = 3). Confidence limits were set: *p = <0.2, **p = <0.05 ***p = <0.005. C. Cells were incubated in the absence or presence of KU0063794, TMZ and KU0063794 or TMZ alone. Cells were incubated with [35S] methionine, as described in Materials and Methods. Incorporation of radioactive methionine into protein was determined as cpm/μg protein; results are presented as a% of methionine incorporated in to cells incubated in the absence of any treatments. Error bars are the S.D (n = 3).

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