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Figure 3 | Molecular Cancer

Figure 3

From: mTOR inhibition and levels of the DNA repair protein MGMT in T98G glioblastoma cells

Figure 3

KU0063794 inhibits protein translation via mTOR kinase inhibition in T98G cells but MGMT protein levels are increased. T98G cells were incubated in the absence (lane 1) or presence of KU0063794 for 12 hours (lane 2), 24 hours (lanes 3), 48 hours (lane 4) or 72 hours (lane 5). A. Western blotting was carried out as described in Materials and Methods B. Aliquots of protein extract were subjected to m7GTP-Sepharose affinity chromatography as described in Materials and Methods. Western blotting was carried out. All lanes were resolved on the same gel. C. T98G cells were incubated in the absence (lane 1) or presence of KU0063794 for 12 hours (lane 2), 24 hours (lanes 3), 48 hours (lane 4) or 72 hours (lane 5). Proteins were visualised by Western blotting using the antiserum indicated. D. MGMT protein levels in (C) were quantified and expressed relative to the α tubulin loading control. Error bars are the SE (n = 3). Confidence limits were set: *p = <0.2, **p = <0.05 ***p = <0.005. E. Cells were incubated in the absence or presence of KU0063794 as indicated. Mitochondrial activity (a measure of cell viability) was assessed using an MTS assay as described in Materials and Methods and is expressed relative to untreated cells (set at 100%). Error bars are the S.D (n = 3).

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