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Figure 2 | Molecular Cancer

Figure 2

From: Expression and Y435-phosphorylation of Abelson interactor 1 (Abi1) promotes tumour cell adhesion, extracellular matrix degradation and invasion by colorectal carcinoma cells

Figure 2

Abi1 expression and subcellular localization in CHD1 colorectal carcinoma cells. A, western immunoblotting of CHD1 whole cell lysate shows expression of Abi1 and hnRNP K as well as a 100/85 kD double-band for Laminin5, but no detectable levels of E-Cadherin. B, IF microscopy shows peripheral, rim-like distribution of Cortactin (I) and Abi1 (II). Signals for both Abi1 and Y435-phosphorylated Abi1 in the cell periphery, but not in the perinuclear region, are extinct after treatment with 10 μM STI571 (III). C, fibronectin adhesion assay shows cellular attachment with lamellipodial protrusions (I) and peripheral positivity for Y435-phosphorylated Abi1 (II) under control conditions, but only few attached cells (III) with condensed actin and negativity for pAbi1 after treatment with 10 μM STI571 (IV). Cell attachment was significantly reduced after treatment with STI571 (V). DMSO, dimethyl sulfoxide. Scale bars as indicated. ***p < 0.001.

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Molecular Cancer

ISSN: 1476-4598