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Figure 3 | Molecular Cancer

Figure 3

From: Expression and Y435-phosphorylation of Abelson interactor 1 (Abi1) promotes tumour cell adhesion, extracellular matrix degradation and invasion by colorectal carcinoma cells

Figure 3

Gelatine-based ECM degradation assay. A, CHD1 cells seeded on Cy3-conjugated gelatine matrix show cytoplasmic and peripheral positivity for Cortactin (I) and Abi1 (II) at the sites of matrix degradation. This is confirmed in 3D surface reconstruction for Abi1 (III, arrow). Application of 10 μM STI 571 leads to loss of Abi1 staining in cell periphery together with lack of gelatine degradation (IV). B and C, Application of STI571 in a concentration of 10 μM (I: DMSO control, II: STI571) as well as Abi1 RNAi knockdown (III: pSuper vector control, IV: Abi1 RNAi) inhibits ECM degradation as shown by a significant reduction in degradation coefficient. DMSO, dimethyl sulfoxide. Scale bars as indicated. ***p < 0.001.

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