Figure 3

CD133 and Slug regulate N- cadherin expression, which is required for migration and invasion. (A) A wound healing assay showed that the healing speed decreased after N-cadherin neutralization using an antibody. (B) Statistical analysis of the mean healed area demonstrated significant differences between with and without N-cadherin antibody neutralization at 12 h (p < 0.05) and 24 h (p < 0.01). (C) N-cadherin mRNA expression decreased by greater than 95% and 60% after CD133 and Slug knockdown, respectively, validated by real-time RT-PCR. (D) N-cadherin protein expression decreased after CD133 or Slug knockdown, as shown by Western blotting. (E) N-cadherin protein expression increased after CD133 was overexpressed in Capan1M9 cells and Panc-1 cells, as shown by Western blot analysis.