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Figure 3 | Molecular Cancer

Figure 3

From: FW-04-806 inhibits proliferation and induces apoptosis in human breast cancer cells by binding to N-terminus of Hsp90 and disrupting Hsp90-Cdc37 complex formation

Figure 3

FW-04-806 induces proteasome-dependent degradation and decreases Hsp90 client protein level. (A) SKBR3 cells were treated by FW-04-806 at 10, 20, 40 μM for 24 h; HER2, phosphorylated HER2 (p-HER2), Raf-1, Akt, and phosphorylated Akt (p-Akt) protein levels were analyzed with western blotting. (B) SKBR3 cells were treated with 20 μM of FW-04-806 for 0, 3, 6, 12, or 24 h; HER2, p-HER2, Raf-1, Akt, and p-Akt protein levels were analyzed with western blot. (C) MCF-7 cells were treated by FW-04-806 at 10, 20, 40 μM for 24 h; western blot detected the protein expression of Raf-1, Akt, and p-Akt. (D) MCF-7 cells were treated with 20 μM FW-04-806 for 0, 3, 6, 12, or 24 h; western blot detected the protein expression of Raf-1, Akt, and p-Akt. (E) SKBR3 was pretreated with 1 μM of MG132 for 2 h in the presence or absence of 20 μM of FW-04-806 for an additional 12 h. Whole-cell lysates were subjected to western blot analysis using antibodies against HER2, Raf-1, Akt, and β-actin. (F) SKBR3 was treated with DMSO or FW-04-806 at 10, 20, 40 μM for 24 h. The total RNA was extracted for quantitative Real-time PCR of AKT, HER2, Raf-1 and Hsp90, using GAPDH as control. *P < 0.05: significant difference from control by analysis of variance; **P < 0.01: very significant difference from control by analysis of variance.

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