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Figure 1 | Molecular Cancer

Figure 1

From: Higher FOXP3-TSDR demethylation rates in adjacent normal tissues in patients with colon cancer were associated with worse survival

Figure 1

Purification of nTregs and iTregs from PBMCs and their identification using FOXP3 -TSDR demethylation-specific qPCR assays. (A) Purification of CD4+CD25+CD127lo nTregs (bottom right) and CD4+CD25-CD45RA+ naïve T cells (upper right) by FACS after separation from PBMCs obtained from a male donor. (B) Representative analysis of the cell purities of FOXP3+ cells in nTregs (93.7%, lower panel) and iTregs (97.2%, upper panel) purified from a CD4+ CD25+ T cell subset by FACS after Treg expansion in vitro. As indicated with black arrows, populations of unstained cells and cells containing IL-2 beads were used as the controls for the quantification of nTregs and iTregs, respectively. (C) The demethylation rates of FOXP3-TSDR varied dramatically between nTregs and iTregs, and the specific primer sets were verified in quantitative real-time PCR.

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