Figure 1
From: Differential chemosensitivity to antifolate drugs between RAS and BRAF melanoma cells
Mutation dependent response to alkylating agents. Nine melanoma cell lines with activating mutations in either BRAF or RAS were treated with serial increasing concentrations of A, carmustine B, cisplatin, C, dacarbazine (DTIC) or D, temozolomide (TMZ). After 5 days, cells were quantified using toluidine blue staining. Untreated cells were set 100% and the GI50 for each drug was calculated as the mean of 3 independent measurements. Each point corresponds to an individual cell line. Student’s t test compares the average GI50 for mutBRAF cell lines vs mutNRAS cell lines. ns = not significant, ***p = 0.0006 for DTIC MutBRAF vs mutRAS cells.