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Figure 2 | Molecular Cancer

Figure 2

From: Long non-coding RNA HOTAIR, a c-Myc activated driver of malignancy, negatively regulates miRNA-130a in gallbladder cancer

Figure 2

The expression changes of c-Myc or HOTAIR after transfection of c-Myc siRNA or pcDNA3.1-c-Myc in GBC-SD cells. The relative mRNA expression levels were evaluated with real-time qPCR. c-Myc protein levels were determined using Western blot assay. Each experiment was performed in triplicate. (A) pcDNA3.1-c-Myc markedly upregulated the expression of c-Myc at mRNA levels. (B) Representative images of western blot results indicated pcDNA3.1-c-Myc significantly upregulated the expression of c-Myc at protein levels. (C) pcDNA3.1-c-Myc significantly upregulated the expression of HOTAIR at mRNA levels. (D) c-Myc siRNA significantly downregulated the expression of c-Myc at mRNA levels. (E)) Representative images of western blot results indicated c-Myc siRNA significantly downregulated the expression of c-Myc at protein level. (F) c-Myc siRNA significantly down-regulated the expression of HOTAIR at mRNA levels. Error bars represent S.E.M., n = 3. *p < 0.05; **p < 0.01.

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