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Figure 3 | Molecular Cancer

Figure 3

From: Long non-coding RNA HOTAIR, a c-Myc activated driver of malignancy, negatively regulates miRNA-130a in gallbladder cancer

Figure 3

c-Myc regulates HOTAIR promoter activity, depending on E-box element. (A) Dual luciferase assay on GBC-SD cells cotransfected with firefly luciferase constructs containing the HOTAIR promoter and pcDNA3.1 or pcDNA3.1-c-Myc. (B) Dual luciferase assay on GBC-SD cells cotransfected with firefly luciferase constructs containing the HOTAIR promoter and c-Myc siRNA or the control siRNA. (C) schematic of the HOTAIR-promoter-luciferase construct is depicted with locations of the E-box element and sequences of point mutation. (D, E) Dual luciferase assay on GBC-SD cells cotransfected with firefly luciferase constructs (mutant at E-box element) and pcDNA3.1-c-Myc (D) or c-Myc siRNA (E). All of the transfection was performed in triplicates. The values are presented as the mean ± S.E.M. of the ratio of firefly luciferase activity to renilla luciferase activity and are representative of at least three independent experiments. Data are shown as the mean ± S.E.M, based on at least three independent experiments. *p < 0.05; **p < 0.01.

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