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Figure 4 | Molecular Cancer

Figure 4

From: Long non-coding RNA HOTAIR, a c-Myc activated driver of malignancy, negatively regulates miRNA-130a in gallbladder cancer

Figure 4

Confirmation of the binding of c-Myc and Max at the promoter region of HOTAIR. (A) c-Myc binding at the promoter region of HOTAIR containing the E-box element and a random region in HOTAIR promoter region (does not contain an E-box, negative control) was assessed by chromatin immunoprecipitation (ChIP). (B) ChIP-derived DNA was amplified by qRT-PCR using specific primers. The levels of qPCR products are expressed as a percentage of input DNA. (C) EMSA showed the interaction of c-Myc with the lncRNA-HOTAIR promoter in vitro. The symbol “*” means the oligonucleotides labled by biotin. Data are shown as the mean ± S.E.M, based on at least three independent experiments. *p < 0.05; **p < 0.01.

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