Identification of miR-130a as a target of HOTAIR. (A) Alignment of potential HOTAIR base pairing with miR-130a as identified by Starbase v2.0 (http://starbase.sysu.edu.cn/mirLncRNA.php). HOATIR (top) consist of 6 exons, where the putative binding site is in exon 6. (B) HOTAIR specific siRNA reduced the endogenous HOTAIR mRNA level. (C, D) Upregulation of miR-130a by si-HOTAIR detected by RT-PCR (C) and northern blot (D). GBC-SD cells were transfected with control siRNA or si-HOTAIR, and total RNA was isolated 48 h after transfection. Error bars represent S.E.M., n = 3. *p < 0.05; **p < 0.01.