Identification of miR-130a as a target of HOTAIR. (A) pcDNA3.1-HOTAIR upregulated the HOTAIR mRNA level. (B, C) Downregulation of miR-130a by ectopic expression of HOTAIR detected by RT-PCR (B) and northern blot (C). GBC-SD cells were transfected with vector control or HOTAIR or mutant HOTAIR, and total RNA was isolated 48 h after transfection. (D) The mutant HOTAIR at putative binding site. Error bars represent S.E.M., n = 3. *p < 0.05; **p < 0.01.