The mechanism of the regulation of miR-130a by HOTAIR. (A) Effect of HOTAIR on mature miR-130a, pri-miR-130a and pre-miR-130a. (B) Pull-down of Ago 2 by biotin-labeled HOTAIR or loc285194 RNA probe, as detected by western blot. The loc285194 lane was composed from the same gel with the same contrast. (C) Detection of miR-130a in the same pellet precipitated by the HOTAIR probe, but not in the pellet precipitated by the loc285194 probe. Error bars represent S.E.M., n = 3. *p < 0.05; **p < 0.01, n.s., not significant.