Figure 3
From: PP2A inhibition overcomes acquired resistance to HER2 targeted therapy
mTOR and eEF2k mediated regulation of eEF2 phosphorylation. (A) Immunoblot analysis of total and phosphorylated mTOR(Ser2448) in SKBR3-par and SKBR3-L cells following 24 hr. lapatinib treatment. (B) Effect of rapamycin on growth of SKBR3-par and SKBR3-L cells. Error bars represent the mean ± SD (n = 3). (C) Immunoblot analysis of total and phosphorylated eEF2(Thr56) following 24 hr. treatment with lapatinib and/or rapamycin. (D) Immunoblot analysis of total and phosphorylated eEF2k(Ser366, 359) in SKBR3-par and SKBR3-L cells following 24 hr. lapatinib treatment. (E) Immunoblot examining the effect of NH125 alone and in combination with lapatinib on the phosphorylation of eEF2(Thr56) in SKBR3-par cells. *denotes p ≤ 0.05.