Figure 3
From: Salinomycin treatment reduces metastatic tumor burden by hampering cancer cell migration
Metastasis formation is reduced by salinomycin treatment in a syngenic intravenous mouse tumor model. A) Tumor growth of 4T1-luc tumors in the lung. 1×105 4T1-luc cells were intravenously injected into 10 female BALB/c mice per group. Animals were treated either with mock (control) or 5 mg/kg salinomycin on day 0, 3, 6 and 9. Tumor growth was monitored for 13 days at indicated time points using bioluminescence imaging. B) Live imaging of 4T1-luc tumors in mouse lungs. Bioluminescence images were taken in dorsoventral (upper panel) and ventrodorsal (lower panel) position and presented for day 13. Signal intensities are displayed as photons/second/cm2 (scale bar). C) Ex vivo imaging of 4T1-luc tumor-bearing lungs. Mice were euthanized on day 13 and the resected lungs of mock- (control) and salinomycin-treated mice were imaged. Signal intensities are displayed as photons/second/cm2. (Student’s t-test, two-tailed; ns = not significant). Metastases in 4T1-luc tumor-bearing mice. D) Brain, E) spleen and F) kidneys were analyzed for metastases using an ex vivo luciferase assay. (Student’s t-test, one-tailed; *p < 0.05; **p < 0.01).