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Figure 2 | Molecular Cancer

Figure 2

From: Anterior gradient protein 2 promotes survival, migration and invasion of papillary thyroid carcinoma cells

Figure 2

Effects of AGR2 knockdown on TPC-1 cell growth, migration and invasion. A) Expression levels of AGR2 in Nthy-ori 3–1 and TPC-1 cells. Cells were lysed and analyzed by Western blotting with the indicated antibodies. B) TPC-1 cells were transfected with AGR2 siRNA or with scrambled siRNA and counted at different time points. Values represent the average of triplicate experiments ± standard deviations. C) TPC-1 cells were transfected with AGR2 siRNA or with scrambled siRNA; after 48 and 72 hours cells were collected by trypsinization, stained for 10 minutes with trypan-blue and counted in triplicate. The percentage of trypan blue excluding cells compared to cells transfected with scrambled siRNA is reported ± standard deviations. D) TPC-1 cells were transfected with AGR2 siRNA or with scrambled siRNA; after 48 hours a wound was introduced and cell migration into the wound was monitored at 12 and 24 hours. E) Wound closure was measured by calculating pixel densities in the wound area and expressed as percentage of wound closure of triplicate areas ± standard deviations. F) TPC-1 (1 × 105) cells were transfected with AGR2 siRNA or with scrambled siRNA; 48 hours after transfection, cells were seeded in the upper chamber of transwells and incubated for 24 hours; the upper surface of the filter was wiped clean and cells on the lower surface were stained, photographed and counted. This figure is representative of three independent experiments. G) Invasive ability is expressed as number of invaded cells. Values represent the average of triplicate experiments ± standard deviations. H) TPC-1 cells were transfected with AGR2 siRNA or with scrambled siRNA. Cells were harvested at different time points and protein lysates were subjected to immunoblotting with the indicated antibodies. Asterisks indicate P < 0.05 (*), P <0.01 (**) and P < 0.001 (***).

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