Figure 4
BSSP4- and T 3 -mediated cell migration occurs via the ERK signaling pathway. The transwell migration (A, C) and invasion assays (B, D) were performed for two BSSP4-expressing (Huh7-BSSP4 #1, 2) and one control (Huh7-BSSP4 #1) cell line (A, B), and J7-TRα1 cells (C, D). Stable lines (105) (A, B) and T3-treated J7-TRα1 (5 × 104) (C, D) cells were treated with DMSO and U0126 (10 μM) added to the upper chamber of Transwell units, followed by incubation for 24 h. Transwell filters were stained with crystal violet in the upper panel, and migration ability quantified in the lower panel. Values are shown as fold increase of Huh7-BSSP4 relative to Huh7-control, and T3 relative to Td. Differences were analyzed using One-way ANOVA, *P < 0.05.