Figure 6
BSSP4 increases cell motility in vitro. Expression of BSSP4 was detected in BSSP4-expressing clones (#1, #2) and controls (control #1, #2) with Western blotting (A). (B) Migration and (C) invasion abilities of two BSSP4-overexpressing and two control cell lines were analyzed using a Transwell assay. Transwell filters were stained with crystal violet in the upper panel, and migration and invasion abilities quantified in the lower panel (B, C). The BSSP4 expression in J7-TR-control cells and J7-TR-BSSP4-KD cells treated with/without T3 was detected by Western blotting (D). The migration ability was determined with a Transwell assay (E, left panel), and the quality index was illustrated in E, right panel. Values are presented as fold increase of J7-BSSP4, relative to J7-control. Differences were analyzed using One-way ANOVA, *P < 0.05.