Skip to main content
Figure 8 | Molecular Cancer

Figure 8

From: Thyroid hormone enhanced human hepatoma cell motility involves brain-specific serine protease 4 activation via ERK signaling

Figure 8

BSSP4 expression is upregulated in vitro and in vivo, and the T/ N ratios of BSSP4 and TR levels are positively correlated in HCC. (A) The BSSP4, TRα/TRβ and P-Erk proteins in HCC specimens were determined using Western blotting. TRs and BSSP4 were overexpressed in 12 representative tumor tissues. The correlations of T/N ratios between BSSP4 and TR (B) and BSSP4 and VEGF (C) were analyzed using linear regression analysis. BSSP4 regulation by T3 at different concentrations (0, 1 and 10 nM) and time-points (24 and 48 h) was examined in J7-TRα1 cells at (D) both mRNA (D, upper panel) and protein (D, lower panel) levels using Q-PCR (D, upper panel) and Western blotting (D, lower panel), respectively. (E) Lung sections of SCID mice injected with J7-TRα1 cells treated with hyper-T3 (hyperthyroid) and administered normal drinking water (euthyroid) were analyzed with IHC (upper panel) and H & E (lower panel) staining to examine BSSP4 expression (upper panel) and tumor foci (lower panel).

Back to article page