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Figure 2 | Molecular Cancer

Figure 2

From: Challenging the roles of CD44 and lipolysis stimulated lipoprotein receptor in conveying Clostridium perfringens iota toxin cytotoxicity in breast cancer

Figure 2

Changes of LSR expression alter iota toxin sensitivity in breast cancer. (A) MCF-7 and MDA-MB-231 cells were stably transfected with either a scrambled control shRNA plasmid (scrambled control-shRNA), or a plasmid containing shRNA specifically targeting LSR variant 1 (LSR-shRNA). Cells were grown to 80% confluence, lysates isolated, and then analyzed via western blot using an LSR-specific antibody and α-tubulin for loading control. Cells were treated with Ia or Ia + Ib for 8 h. Representative images demonstrate cell rounding and detachment from tissue culture dish, indicative of cell death. (B) Hs578t and SUM159 cells were stably transfected with either a control plasmid (pCMV), or a plasmid containing the full-length gene for LSR variant 1 (LSR+). Cells were grown and treated as stated in (A). A minimum of three independent experiments was performed for each analysis.

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Molecular Cancer

ISSN: 1476-4598