Challenging the roles of CD44 and lipolysis stimulated lipoprotein receptor in conveying Clostridium perfringens iota toxin cytotoxicity in breast cancer

Table 3 Iota toxin sensitivity in LSR overexpressing and knockdown cells

	Cell line	Ia (10 ng/ml)	Ia (10 ng/ml) + Ib (20 ng/ml)	Ia (25 ng/ml) + Ib (50 ng/ml)	Ia (50 ng/ml) + Ib (100 ng/ml)	Ia (100 ng/ml) + Ib (200 ng/ml)
Overexpressing	SUM1315mo-CMV	-	-	-	-	+
	SUM1315mo-CD44+	-	-	-	-	-
	SUM1315mo-LSR+	-	-	+	+	++
	SUM159-CMV	-	-	-	-	-
	SUM159-LSR+	-	-	-	-	-
	Hs578t-CMV	-	-	-	-	-
	Hs578t-LSR+	-	-	-	+	+
Knockdown	MCF-7 Scrambled Control	-	+	+	++	++
	MCF-7 LSR shRNA	-	+	+	+	+
	SUM1315mo Control	-	+	+	+	+
	SUM1315mo-LSR shRNA	-	-	-	-	-
	MDA-MB-231 Scrambled Control	-	-	-	+	+
	MDA-MB-231 LSR shRNA	-	-	-	-	-

(−) Resistant, 0 to 10% cell rounding; (+) Sensitive, 11 to 50% cell rounding; (++) Highly Sensitive, >50% cell rounding. Toxin Sensitivity at 8 h post treatment.

ISSN: 1476-4598