Aldolase activation of the Wnt pathway depends on the “β-catenin degradation complex”. (A) SW480 cells were co-transfected with the following plasmids: GFP-ALDOC, GFP-ALDOB or an empty vector, pTOPFLASH or pFOPFLASH and Renilla. Forty eight hours later, cells were harvested and lysates were measured for their luciferase activity, (upper panel) and levels of endogenous active β-catenin by western blot (lower panel). Tubulin served as a loading control. Data presented describe relative mean values and standard deviations of four independent experiments performed in duplicates or triplicates. (B) HEK293T cells were transfected with GFP, GFP-β-catenin, GFP-ALDOC or the GFP-ALDOB constructs as indicated, along with the pTOPFLASH or pFOPFLASH and β-gal plasmids. Luciferase assay (upper panel) and western blot analysis (lower panel) were performed as described.