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Figure 4 | Molecular Cancer

Figure 4

From: Aldolase positively regulates of the canonical Wnt signaling pathway

Figure 4

Aldolase requires GSK-3β for activating the Wnt pathway but does not affect the phosphorylation of β-catenin. (A) HEK293T cells were subjected to GSK-3β depletion using siGSK-3β RNA oligonucleotides. Twenty four hours later, cells were co-transfected with GFP-ALDOC, GFP-ALDOB or an empty vector. After additional forty eight hours, cells were harvested and separated by SDS-PAGE. Blots were incubated with anti-active β-catenin, anti-GFP, anti-GSK-3β or anti-tubulin as indicated (upper panel). TINA analysis of active β-catenin band intensity is presented (lower panel). (B) Untransfected or cells transfected with ALDOB or ALDOC were treated with SB (10 μM; 4 hours) as indicated. Twenty four hours later the cells were harvested and separated by SDS-PAGE. Blots were incubated with the indicated antibodies (upper panel). TINA analysis of active b-catenin band intensity was performed on 2 separate experiment (lower panel). (C) HEK293T or HeLa cells were transfected with GFP-ALDOC, GFP-ALDOB or an empty vector. Forty eight hours later, cells were harvested and lysates were subjected to western blot analysis using specific antibodies to anti-phospho-β-catenin, anti-GFP, anti-GSK-3β or anti-tubulin as indicated.

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