Figure 6

HMGB1 regulated docetaxel-induced autophagy in docetaxel-resistant LAD cells. (A) Whole cell lysates, nuclear exacts and cytoplasmic fractions from SPC-A1/DTX and H1299/DTX cells were treated with indicated doses of docetaxel in the presence or absence of EP(10 mM, 1 h) were subjected to western blot analysis for LC3, p62 and HMGB1. (B) Both docetaxel-resistant cells were transfected with HMGB1 shRNA before treatment with the indicated doses of docetaxel for 48 h. Total cell lysates, nuclear extracts, cytoplasmic fractions were subjected to western blot analysis for LC3, p62 and HMGB1. (C) Both docetaxel-resistant cells were co-transfected with either control or HMGB1 shRNA and GFP-LC3 plasmid followed by treatment with docetaxel (100 μg/l) for an additional 24 h. Bar = 50 μm. Values are reported as mean ± S.D. of three independent experiments. *P < 0.05, **P < 0.01.