Figure 8
From: HMGB1-mediated autophagy promotes docetaxel resistance in human lung adenocarcinoma
Suppression of HMGB1 resensitized docetaxel-resistant SPC-A1/DTX cells to docetaxel in vivo. (A) NOD/SCID mice were inoculated with 5.0 × 106 SPC-A1/DTX cells following transfection of control or HMGB1 shRNA and treated with docetaxel (1 mg/kg) beginning at day 6. Tumor volumes were calculated for 14 days. (n = 5; *P < 0.05). (B) Representative photographs of tumors formed at 2 weeks after subcutaneous transplantation are shown. (C) Hematoxylin and eosin (H&E)-stained, proliferating cell nuclear antigen (PCNA)-stained and TUNEL assay stained sections of the transplanted tumors are shown (original magnification, ×400). (D) HMGB1 and LC3 expression in the transplanted tumors were determined by western blot analysis.