Increased proliferation and upregulated Wnt- and Notch-Signaling contributes to the increased tumorigenesis in APC-Cldn1 mice. (A) Tumors from APCMin and APC-Cldn1 mice were immunostained using anti-Ki67 antibody to quantitate the proliferation, (n = 9 APC, n = 10 APC-Cld-1mice) p = 0.0125. (B) Activation of Wnt signaling was determined by immunostaining for β-catenin using anti-β-catenin antibody. The nuclear/cytoplasmic β-catenin staining is increased in APC-Cldn1 mice tumors compared to APCMin mice. (C) qRT-PCR analysis of Wnt target genes and (D) Notch target genes in tumors of APCMin and APC-Cldn1 mice (n = 3 mice each group). Values are mean ± S.E.M. *p < 0.05.