Table 4 Summary of previous studies on BRAF mutation in colorectal carcinoma
From: A very low incidence of BRAF mutations in Middle Eastern colorectal carcinoma
Study | Country | % with BRAF | BRAF mutation detected | Methods of mutation detection |
|---|---|---|---|---|
Li-Ling H, 2012 | Taiwan | 1.1 | 2/182 | High-resolution melting point (HRM) polymerase chain reaction (PCR) for BRAF V600E mutations |
Li H , 2011 | China | 7 | 14/200 | Sequenced by Pyrosequencer PyroMark ID system-Exon 15, V600E |
Liou J, 2011 | China | 3.8 | 12/314 | Sequencing of exons 11 and 15 |
Yokota T, 2011 | Japan | 4.7 | 15/319 | Cycleave PCR technique for V600E mutation |
Nakanishi R, 2012 | Japan | 6.7 | 17/254 | BRAF pyrokit pyrosequencing |
Ajay Goel, 2009 | Israel | 18.7 | 24/128 | Direct sequencing using the BigDye version 1.1Â cycle-sequencing kit |
Rozek L, 2010 | Israel | 5 | 65/1300 | Direct sequencing of exon 15 of BRAF |
Price T, 2011 | Australia | 10.6 | 33/315 | High-resolution melting point (HRM) polymerase chain reaction (PCR) for BRAF V600E mutations |
Tran B, 2011 | Australia | 11 | 57/524 | Mutation-specific real-time polymerase chain reaction assay. |
Di Nicolantonio, 2008 | Italy | 10 | 11/113 | Automated sequencing by ABI PRISM 3730 exon15 |
Richman S, 2009 | UK | 7.9 | 422/711 | Pyrosequenced on a PyroMark ID system (Biotage AB) Codon 600 |
Roth A, 2009 | Switzerland | 7.9 | 103/1307 | Allelic discrimination assay on a 7500 real-time polymerase chain reaction for V600E |
Zlobec I, 2010 | Switzerland | 12 | 45/374 | BRAF (exon 15 codon 600) direct sequencing of single-stranded PCR products using the BigDyeVR Terminator v1.1Â cycle sequencing kit |
Farina-Sarasqueta A, 2010 | Netherlands | 19.8 | 59/297 | V600E mutation on the BRAF gene by real-time PCRLight Cycler v2.0 (Roche) |
Saridaki Z, 2010 | Greece | 8.3 | 12/144 | Real-time PCR using the allelic discrimination method with ABI PRISM 7900Â T Sequence Detection System |
Modest D, 2012 | Germany | 11.6 | 17/146 | Exons 11 and 15 of the BRAF pyro-sequencing |
Samowitz W, 2005 | USA | 9.5 | 87/911 | Sequencing in both directions |
Shaukat A,2010 | USA | 21.8 | 36/165 | BRAF V600E mutation -Mutector II BRAF/Ras mutation detection panel assay kit |
Kalady M,2012 | USA | 12 | 56/475 | Direct sequencing |
Ogino S,2012 | USA | 15 | 11/75 | c.1799 T > A (p.V600E) mutation |