Figure 1

HUVECs internalize LAMA84 exosomes. a: Analysis at confocal microscopy of HUVECs treated, for 1 hour and 4 hours, with 20 μg/ml (Exo 20 μg/ml) and 50 μg/ml (Exo 50 μg/ml) of LAMA84 exosomes, compared with untreated HUVECs (Control). HUVECs were stained with phalloidin Alexa Fluor (green), nuclear counterstaining was performed using Hoescht (blue), exosomes were labelled with PKH26 (red). To evaluate whether exosomes uptake was mediated by endocytosis in an energy-dependent process, HUVECs treated with 20 μg/ml (Exo 20 μg/ml) and 50 μg/ml (Exo 50 μg/ml) of LAMA84 exosomes were incubated at 4°C, for 1 hour and 4 hours and compared with untreated HUVECs. b: Analysis at confocal microscopy of HUVECs treated, for 1 hour, with 50 μg/ml of exosomes (Exo 50 μg/ml) and EIPA (50 μM), compared with control cells (Control). Scale bar = 10 μm.