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Figure 6 | Molecular Cancer

Figure 6

From: Exosomal shuttling of miR-126 in endothelial cells modulates adhesive and migratory abilities of chronic myelogenous leukemia cells

Figure 6

miR-126 delivered by exosomes modulates LAMA84 migration, adhesion and transendothelial transmigration. a: Effects of conditioned medium (CM) of HUVECs pretreated with 10 μg/ml 20 μg/ml and 50 μg/ml of exosomes on LAMA84 cells motility compared with CM from untreated HUVECs. LAMA84 cells motility was determined towards conditioned medium of HUVECs transfected with miR-126 inhibitor (2’-O-Me-miR-126) and treated with 10 μg/ml (Exo 10μg/ml, 2’-O-Me-miR-126), 20 μg/ml (Exo 20μg/ml, 2’-O-Me-miR-126) and 50 μg/ml (Exo 50 μg/ml, 2’-O-Me-miR-126) of LAMA84 exosomes compared with untransfected cells. MiScript Inhibitor Negative Control (Scramble) was used as a negative control of miR-126 inhibitor transfection. LAMA84 cells motility towards CM of HUVECs transfected with: miR-126 mimic and treated with 10 μg/ml (Exo 10μg/ml, miR-126 mimic), 20 μg/ml (Exo 20 μg/ml, miR-126 mimic) and 50 μg/ml (Exo 50μg/ml, miR-126 mimic) of LAMA84 exosomes compared with untransfected cells. AllStars Negative Control (Scramble) was used as a negative control of miR-126 mimic transfection. Values are expressed as Fold of Increase (FOI). Values are the mean ± SD of 5 fields in 3 independent experiments *p 0.05; **p 0.01. b: Adhesion of LAMA84 cells to HUVECs treated, for 6-12-24 hours, with 20 and 50 μg/ml of LAMA84 exosomes compared with control HUVECs. c: Adhesion of LAMA84 cells to HUVECs treated with 20 and 50 μg/ml of LAMA84 exosomes compared with control HUVECs. The adhesion of LAMA84 cells was evaluated on HUVECs transfected with miR-126 mimic and then treated with 20 and 50 μg/ml of LAMA84 exosomes. d: The adhesion of LAMA84 cells was evaluated in HUVECs transfected with miR-126 inhibitor and then treated with 20 and 50 μg/ml of LAMA84 exosomes. e: HUVECs were grown as a monolayer and treated with 10, 20, 50 μg/ml of LAMA84 exosomes. After 6-12-24 hours of treatment, we evaluated the transendothelial migration of LAMA84 cells.

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