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Figure 1 | Molecular Cancer

Figure 1

From: Ponatinib efficiently kills imatinib-resistant chronic eosinophilic leukemia cells harboring gatekeeper mutant T674I FIP1L1-PDGFRα: roles of Mcl-1 and β-catenin

Figure 1

Ponatinib inhibits phosphorylation of PDGFRα and its downstream signaling molecules. (A) BaF3-T674I FIP1L1-PDGFRα cells exhibited differential sensitivity to ponatinib and sorafenib. BaF3-T674I FIP1L1-PDGFRα cells were treated with the TKIs at the indicated concentrations for 24 h, and the levels of phosphorylated and total PDGFRα were detected with the relevant antibodies. (B) Ponatinib inhibited phosphorylation of PDGFRα in a concentration-dependent manner. EOL-1 and BaF3-WT or -T674I FIP1L1-PDGFRα cells were exposed to escalating concentrations of ponatinib for 24 h. (C) Ponatinib inhibited phosphorylation of PDGFRα in a time-dependent manner. The concentrations of ponatinib were 1 nM for EOL-1, 300 nM for BaF3-WT and -T674I FIP1L1-PDGFRα cells, respectively. (D) Ponatinib concentration-dependently inhibited phosphorylation of Stat3, Stat5, Akt and Erk1/2. The cells were exposed to increasing concentrations of ponatinib for 24 h. (E) Ponatinib time-dependently inhibited phosphorylation of Stat3, Stat5, Akt and Erk1/2. 300 nM ponatinib was applied.

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Molecular Cancer

ISSN: 1476-4598