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Figure 1 | Molecular Cancer

Figure 1

From: HOXA9 promotes homotypic and heterotypic cell interactions that facilitate ovarian cancer dissemination via its induction of P-cadherin

Figure 1

Knockdown of HOXA9 inhibits aggregation and survival of ascitic EOC cells and decreases EOC cell implantation and invasiveness in i.p. xenograft models. Female nude mice (n = 5 per group) were inoculated i.p. with equivalent numbers (2 × 106) of GFP-expressing HOXA9+ control (empty vector, non-targeting) and HOXA9-knockdown (shA9-A, shA9-B) SKOV3ip cells. Mice were sacrificed at 3 weeks thereafter. (A,B) Ascitic cells were collected and stained with Hoechst dye to visualize nuclei and with 7AAD to assay cell death. (A) Representative examples of Hoechst staining (shown in blue) of ascitic cells where GFP-expressing tumor cells (shown in green) were visualized by fluorescence microscopy. Bar, 50 μm. (B) Flow cytometric analysis of 7AAD staining within the gated population of GFP + ascitic tumor cells. (C,D) Representative examples of HE-stained sections of (C) bowel tissues with mesenteric implants (bar, 1 mm) and (D) diaphragmatic implants (bar, 100 μm).

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