Figure 1

MiR-99a/100 ~ 125b cluster host genes in hematopoietic cells and leukemia. A) Genomic architecture of the miR-99a/let-7c/miR-125b-2 (hsa21) and miR-100/let-7a-2/miR-125b-1 (hsa11) cluster. MIR99AHG (alias MONC) and MIR100HG represent the lincRNA host genes of the miRNA cluster, TSSs were determined by 5’RACE-PCR [15]. B) Transcript quantification of MONC and MIR100HG by qRT-PCR in sorted CD34⁺ HSPCs, CD36⁺/ GlyA⁺ erythroid cells, CD41⁺/ CD42b⁺ megakaryocytes, CD15⁺/ CD66b⁺ neutrophil granulocytes, CD14⁺ monocytes, CD3⁺/ CD4⁺/ CD8⁻ and CD3⁺/ CD4⁻/ CD8⁺ T-cells, respectively, CD56⁺/ CD3⁻ NK cells and CD19⁺/ CD3⁻/ CD56⁻ B-cells (left panel; n = 5 each) as well as indicated cell lines (right panel). The B2M gene was used as reference; A.U., arbitrary units. C) Correlation plots and statistics of MONC and D) MIR100HG expression with their cluster miRNA expression in NB4, NOMO-1, THP-1, Kasumi-1, Jurkat, K562, M-07e, Meg-01, CMK and CMY cells measured by qRT-PCR. (B-D) Data are presented as mean ± s.d.