Figure 4
From: LincRNAs MONC and MIR100HG act as oncogenes in acute megakaryoblastic leukemia
Design and evaluation of a lentiviral lncRNA overexpression vector. A) Schematic vector map. PCR primers used in B) are indicated. B) DNA electrophoresis gel of control PCRs validating the lncRNA expression and termination. Primer pair fwd1-rev1 demonstrates genomic integration of transduced cells, pair fwd2-rev1 indicates functional PolyA signal upon product absence in cDNA samples, and pair fwd2-rev2 detects specifically the MONC transcript. For all primer combinations plasmid DNA (vector plasmid, MONC plasmid) was used as a control for respective genomic or cDNA samples. Vector, empty LeGO-CeB/lnc. right graph: qRT-PCR quantification of MONC in transduced HT1080 cells (Data are presented as mean ± s.d. *P < 0.05; **P < 0.01).