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Figure 4 | Molecular Cancer

Figure 4

From: Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma

Figure 4

Inhibition of G9a induces the DUSP4-ERK-mediated autophagy mechanism in HNSCC cells. (A) Lysates from control or G9a-knockdown cells of FaDu or SAS cells were subjected to western blot analysis for examination of autophagy-related signaling pathways. (B) Quantitative real-time PCR analysis of G9a and DUSP4 expression in control or G9a-knockdown FaDu or SAS cells. a, G9a expression in G9a-knockdown cells compared with shLuc-containing lentivirus-infected controls. b, DUSP4 expression in G9a-knockdown cells compared with shLuc-containing lentivirus-infected controls, p < 0.01. (C) Quantitative real-time PCR analysis of DUSP4 expression in controls or BIX-01294 treated FaDu or SAS cells for 24 h. a, DUSP4 expression in SAS cells treated with 2.5 or 5 μM BIX-01294 compared with untreated controls. b, DUSP4 expression in FaDu cells treated with 2.5 or 5 μM BIX-01294 compared with untreated controls, p < 0.05. (D) The spearman correlation between G9a and DUSP4 mRNA expression of tumors from 20 HNSCC patients. (E) Cell lysates from FaDu cells expressing a combination of inducible-knockdown of G9a, transient knockdown of DUSP4 and 10 μM U0126 treatment were subjected to western blot to analyze the indicated proteins. (F) The clonogenic cell survival assay of FaDu cells expressing a combination of inducible-knockdown of G9a, transient knockdown of DUSP4 and 10 μM U0126 treatment. Significant differences were determined between the groups (***, p < 0.001).

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