Nicotine and EGF induces expression of STMN3 and GSPT1 in NSCLC cell lines; and depletion of ID1 by siRNA reduced this induction. (A & B) RT-PCR showing significant fold reduction in STMN3 and GSPT1 in A549. (C & D) Similar results were obtained in H1650 cells, (E) Western blot analysis showing the upregulation of STMN3 & GSPT1 proteins by nicotine and EGF in non-targeting control siRNA cells and absence of induction in ID1 depleted A549 cells, (F) similar results were obtained in H1650 cells. The above data are expressed as mean ± SD of three independent experiments. *represents p value of <0.05; **represents p value of <0.02.