Depletion of STMN3 and GSPT1 by siRNA transfection in NSCLC cells significantly reduces nicotine and EGF induced cell proliferation and invasion. (A & B) showing reduced cell proliferation of A549 cells in response to nicotine and EGF, when STMN3 and GSPT1 are depleted, (C & D) Similar results were obtained in H1650 cells. The results are representative of three independent experiments done in duplicates. (E & F) Depletion of STMN3 and GSPT1 inhibits the invasion of A549 cells upon stimulation with nicotine and EGF as compared to serum starved cells, (G & H) similar inhibition was observed in the H1650 cells. The above data are expressed as mean ± SD of three independent experiments. *represents p value <0.05 and **represents p value < 0.0005.