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Figure 5 | Molecular Cancer

Figure 5

From: Nicotine-mediated invasion and migration of non-small cell lung carcinoma cells by modulating STMN3 and GSPT1 genes in an ID1-dependent manner

Figure 5

Stable transfection confirming the ID1 overexpression in A549 cell and dose dependent induction of STMN3 & GSPT1 by ID1 and depletion of ID1 elevates the expression of STMN3 & GSPT1. (A) The overexpressing (OE) clones showed upregulation of STMN3 and GSPT1 in ID1 over expressing clones as compared to the non-targeting control vector (pcDNA3) as observed by RT-PCR, (B) ID1 protein levels were also elevated as seen in a western blot in the selected clones using G418 resistance confirming the ID1 over-expression, (C) ID1 could induce STMN3-luciferase and GSPT1-luciferase promoters in a dose dependent manner in transient transfection experiments in A549 cells, (D) and H1650 cells as shown in the graph.

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Molecular Cancer

ISSN: 1476-4598