Role of NRSF and ZBP89 in modulating the expression of STMN3 and GSPT1. (A) Depletion of NRSF or ZBP89 leads to the induction of STMN3 and GSPT1 in A549 cells, as seen by a RT-PCR experiment. (B) Similar results were obtained in H1650 cells. (C) Western blot showing the induction of ZBP89 and NRSF upon depletion of ID1 in A549 cells; there was a corresponding decrease in the levels of STMN3 and GSPT1. (D) A western blot on H1650 cells showed essentially identical results.