Depletion of NRSF or ZBP89 leads to an induction of STMN3 and GSPT1. (A) A transient transfection experiment on A549 cells showing that depletion of NRSF leads to an induction of STMN3-Luc, comparable to the induction by co-transfecting ID1; there was no further induction when ID1 was co-transfected with NRSF siRNA. (B) In a similar co-transfection experiment on A549 cells, depletion of ZBP89 led to an induction of GSPT1, and there was no further induction when ID1 was co-transfected. (C) and (D) show similar results that were obtained in H1650 cells. The above data are all expressed as mean ± SD of three independent experiments. *represents p value of <0.05; **represents p value of <0.02.